Description
Aviva Systems Biology Tfap2b ELISA Kit (Mouse) (OKEH06581) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Tfap2b antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Tfap2b and incubated. The Tfap2b found in the sample or standards competes with the biotinylated Tfap2b for limited binding sites on the immobilized anti-Tfap2b antibody. Excess unbound biotinylated Tfap2b and sample or standard Tfap2b is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Tfap2b captured in the well and inversely proportional to the amount of Tfap2b which was contained in the sample or standard